Fluorescence–Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark–Field Hyperspectral Microscopy
M. A. Kryuchkova, I. R. Ishmukhametov, Y. A. Frank, A. V. Simakova, V. V. Yartsev, D.A. Nadueva, A. A. Varenitsina, Y. V. Andreeva, and R. F. Fakhrullin
Том 86 №3
170 просмотров;
Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently–labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark–field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues. Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently–labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark–field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.